Asian Journal of Research in Chemistry

  • Year: 2010
  • Volume: 3
  • Issue: 2

Low-Level Determination of Residual 4-Bromo Methyl- 2′-Cyanobiphenyl in Valsartan by Liquid Chromatography-Mass Spectrometry

  • Author:
  • M Yogeshwar Reddy1,3,, V Ramesh2, Ch Kista Reddy3, N Venugopal1, G Saravanan1, Y Suresh1, M Suryanarayana1, Debashish Datta1, B Raju2
  • Total Page Count: 4
  • DOI:
  • Page Number: 407 to 410

1Matrix Laboratories Ltd., R and D centre, Jinnaram Mandal, Medak, 502325, India

3Department of Chemistry, P.G. College of Science, Saifabad, Hyderabad, India

2National Centre for Mass Spectrometry, Indian Institute of Chemical Technology, Hyderabad, 500 007, India

Abstract

A Liquid chromatographic (LC) method using mass spectrometric (MS) detection was developed and validated for the trace analysis (ppm level) of 4-Bromo methyl- 2′-Cyanobiphenyl in Valsartan drug substances. LC analysis of 4-Bromo methyl- 2′-Cyanobiphenyl was done on Necleosil C18 (100 mm x 4.6 mm, 3 μm) column and the mobile phase is in the ratio of 50:50:1 containing water, acetonitrile, and acetic acid. The flow rate was 1.0 mL min−1 and the elution was monitored at 225 nm. The method was validated as per International Conference on Harmonization (ICH) guidelines. LC-MS is able to quantitate up to 1.0 ppm of 4-Bromo methyl- 2′-cyanobiphenyl.

Keywords

Liquid chromatography, mass spectrometry Valsartan