Development and Validation of Nitazoxanide by HPTLC in Tablet Dosage Form

A simple, precise, accurate, rapid and reproductive HPTLC procedure was developed for development and validation of Nitazoxanide in tablet dosage form at a single wavelength. The mobile phase used was a combination of chloroform: methanol (8:2 v/v) with 15 min time of saturation with filter paper with plate equilibrium. The detection of the combined dosage form was carried out at 330nm using 20 ×10 cm silica gel 60 f 254 TLC plate in the form of 8mm band with 14.5mm interval and migration distance allowed was 70mm. linearity was obtained in the concentration range of 5-50ug/ul of Nitazoxanide with correlation coefficients of 0.9949. The results of the analysis were validated statistically and recovery studies confirmed the accuracy of the proposed method.