A simple, economical, precise and rapid visible spectrophotometric method has been developed for the determination of Serratiopeptidase in tablet dosage forms. Developed method is based on the formation of stable colored complex of drug with Biuret reagent. A wavelength maximum was found to be 531 nm. Linearity was observed in the concentration range of 0.5–5 mg/ml. Correlation coefficient was found to be 0.9993. The result of analysis has been validated statistically and also by recovery study.
Serratiopeptidase, Visible spectrophotometric method, Biuret reagent
