Simultaneous Determination of Ciprofloxacin Hydrochloride and Dexamethasone in Ophthalmic Solution by Reversed Phase High Performance Liquid Chromatography

A gradient reverse phase HPLC method was developed and validated for simultaneous estimation of ciprofloxacin hydrochloride and dexamethasone in pharmaceutical dosage form i.e. ophthalmic solution using Nucleosil C18 (15cm x 4.6mm, 5μm) column with a flow rate of 1.2 ml/minute and detection of 265nm. The mobile phase A consisted of 50 milli molar citric acid and potassium phosphate buffer. The mobile phase B consisted of 100% v/v acetonitrile. The method was validated for system suitability, linearity, accuracy and precision. The system suitability parameters like resolution, theoretical plates and tailing factor were evaluated. The linear range for ciprofloxacin was 3 μg/ml – 55 μg/ml and for dexamethasone was 1 μg/ml – 16 μg/ml. The method has been successfully used to analyze pharmaceutical dosage form i.e. ophthalmic solution containing ciprofloxacin hydrochloride and dexamethasone with good recoveries.