Simultaneous Spectrophotometric Estimation of Curcuminoids and Ascorbic Acid in Bulk Drug and Ayurvedic Polyherbal Tablet Dosage Form

In the present study, an attempt has been made to develop an analytical method for the simultaneous estimation of curcuminoids and ascorbic acid in commercially marketed ayurvedic polyherbal formulation Nisha Amalaki vatti by spectrophotometric method. Simultaneous equations (Vierodt's method) were performed by UV/Visible spectrophotometric. Curcuminoids has absorbance maxima at about 447nm and Ascorbic acid maxima at about 247nm in methanol. The linearity was obtained in the concentration range of 10–50 mcg/mL for both curcuminoids and ascorbic acid. The results were of the analysis were validated statistically and the recovery studies were carried out as per ICH guidelines.