A simple Reverse Phase liquid chromatographic(HPLC) method for the determination of Levetiracetam in human plasma has been developed. Separation was achieved on a Prontosil C8 (150 x 4.6 mm, 5 μm) column with mobile phase composition of [pH 3.8 buffer: acetonitrile] (Solution A): HPLC grade water (Solution B) in the ratio 60:40 v/v with ultra violet detection at 210nm. Lower limit of quantitation was 200ng/ml. Maximum between-run precision was 5.91%. Mean extraction recovery was found to be 100.02 to 103.06%. Stability study showed that after three freezethaw cycles the loss of three quality control samples were less than 10%. Samples were stable at room temperature for 48h and at −20° for 2months. Before injecting onto HPLC system, the processed samples were stable for at least 6h.The method was used to perform bioequivalence study in human.
