1Prof. of Inorganic and Analytical Chemistry, Faculty of Science, Al-Azhar University, Cairo, Egypt
2Forensic Chemist, Medico-Legal Organization, Ministry of Justice, Cairo, Egypt
*Corresponding Author E-mail: ashraf_m1974@yahoo.com; chemistashraf2010@hotmail.com
Online published on 6 March, 2013.
A new, rapid and sensitive reverse phase HPLC method was developed and validated for the determination of amitriptyline hydrochloride in tablet formulations and urine. The mobile phase used acetonitrile and water, (50% v/v) adjust pH to 5 using phosphoric acid. The separation was achieved on C18 reversed-phase column (250 mm x 4 mm i.d.). The flow rate was 0.6 ml/min and UV detection at 254nm. The retention time for amitriptyline hydrochloride was 7.3 min. The calibration curve was linear in the range 0.5–3 μg/mL. The mean recovery for amitriptyline hydrochloride is 100.025%. The assay was precise within day and between days. The method provided excellent sensitivity, recovery, accuracy and reproducibility in therapeutic or toxic concentrations. Common excipients do not interfere.
Amitriptyline hydrochloride, Reverse phase HPLC, C18 reversed-phase column
