Synthesis of Pyrazolopyrimidine Derivatives and Their Antibacterial Activity

A novel series of substituted pyrazolopyrimidine derivatives have been synthesized from substituted hydrazine derivatives and ethoxy (methylene)malanonitrile gives an intermediate (5-amino-1-substituted phenyl pyrazole-4carbonitrile) (1) which on further react with formic acid, acetic acid/HCl and formamide gives series of (1-substituted phenyl)1H pyrazole [3,4-d] pyrimidine-4(5H)-one (2). The synthesized compounds were characterized on the basis of IR, ¹H NMR and mass spectral data. Furthermore, these newly synthesized compounds were screened for their antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa. Compounds PP-6A, PP-2B, PP3B and PP-5C showed significant antibacterial activity.