Asian Journal of Research in Chemistry

The objective of the study is to develop a reversed phase high performance liquid chromatographic method using ultra violet detection for the determination of Dobutamine HCl in bulk and pharmaceutical dosage forms. Phenomenex Luna C₁₈ column (250 mm x 4.6 mm, 5 μ) was used as the stationary phase with a mixture of Acetonitrile:Methanol:Tetrahydrofuran (70:20:10 v/v/v) as the mobile phase. The response of the drug was linear in the concentration range of 10–30 μg/ml. Limit of detection and Limit of Quantification were found to be 0.34 and 1.1 μg/ml, respectively. The percentage recovery ranged between 99.54 and 100.06%. The factors affecting column separation of the analyte were studied. The results demonstrated that this method is reliable, reproducible and suitable for routine analysis.