Synthesis and Antimicrobial Activity of Mannich Bases of 1-Isonicotinoylpyrazolidine-3, 5-Dione

1-isonicotinoylpyrazolidine-3, 5-dione (1) has been synthesized by the condensation of isonicotinohydrazide, diethyl malonate and a catalytic amount of glacial acetic acid. A number of 1-isonicotinoylpyrazolidine-3, 5-dione derivatives have been synthesized by the reaction of (1) with various primary as well as secondary amines in the presence of formaldehyde. The reactions have been carried out following conventional procedure and also under microwave irradiation. The microwave-assisted reactions are rapid, safe, high yielding and superior to conventional methods. The structures of all the products are characterized by micro analytical data and spectroscopic techniques. All the synthesized products are screened for their antibacterial activity against E. coli, P. aeriginosa, B. subtillis S. aureus and C. albicans, A. niger.