Asian Journal of Research in Chemistry
  • Year: 2011
  • Volume: 4
  • Issue: 5

Analytical Methods Validation for Estimation of Squalene from the Rajgeera Seed (Amaranth paniculata)

  • Author:
  • Omprakash H. Nautiyal1,, Krishna K. Tiwari2, Rekha S. Singhal3
  • Total Page Count: 5
  • Page Number: 770 to 774

1ICT and Professor of Organic Chemistry & Natural Products Chemistry, Lovely Professional University, Chaheru, Jalandhar GT Road, Phagwara, Punjab-144402, India

2Visiting Professor (JUET), formerly Professor at Institute of Chemical Technology (UDCT), N.M. Parekh Road, Matunga (E), Mumbai-400019, India

3Professor of Foods, Institute of Chemical Technology (UDCT), Department of Foods and Fermentation, N. M. Parekh Road, Matunga (E), Mumbai-400019, India

*Corresponding Author E-mail: opnautiyalus@yahoo.com, om.prakash@lpu.co.in

Online published on 6 March, 2013.

Abstract

Rajgeera seed (Amaranth paniculata) is now researched as rich source of squalene, earlier source reported was shark liver oil. Squalene was obtained by extraction with SFE and conventional techniques. The Squalene was estimated in the extracted oil using various techniques like UV-Visible spectrophotometer, Iodometric titration, Fourier transform Infra-red spectrophotometer and Gas chromatography. All the analytical techniques were validated by establishing calibrated graph. Out of all these gas chromatography was found to be fast, accurate and reproducible for the estimation of squalene.

Keywords

Amaranth paniculata, analysis, squalene, quality, chromatography, sophisticated analytics