1P.G. Department of Quality Assurance, Institute of Pharmaceutical Education and Research, Borgaon (Meghe), Wardha-442001, Maharashtra, India
2Dr. Vedprakash Patil Pharmacy College, Aurangabad-431005, Maharashtra, India
*Corresponding Author E-mail: vilasarsul@rediffmail.com
Online published on 6 March, 2013.
A simple, precise, accurate, rapid and reproductive HPTLC procedure was developed for development and validation of phyllanthin in tablet dosage form at a single wavelength. The mobile phase used was a combination of Toluene-Ethyl acetate [9:1 V/V]. The detection of the combined dosage form was carried out at 200nm. The Rf value of standard phyllanthin was 0.83 ± 0.04 and matches to the phyllanthin present in polyherbal tablet. The phyllanthin content of tablet was determined and it was found to be 0.178% w/w ±0.0016. The results of the analysis were validated statistically and recovery studies confirmed the accuracy of the proposed method.
Polyherbal tablets, phyllanthin, HPTLC, recovery
