Simultaneous Spectrophotometric Estimation of Formoterol Fumarate and Budesonide in Combined Dosage Form

Asimple, accurate, cost effective and reproducible spectrophotometric method; Q Analysis (Absorbanceratio), has been developed for the simultaneous estimation of formoterol fumarate and Budesonide in rotocap dosage form. Method involves formation of ‘Q-absorbance Equation’ at 257.0 nm (λmax of formoterol fumarate) and 278.0 nm (isoabsorptive point) in distilled water. Beer's law were obeyed (at isoabsorptive point) in the concentration range of 2–20μg/ml for formoterol fumarate having line equation y=0.04950x+0.03520 with correlation coefficient of 0.99987 and 4–40μg/ml for Budesonide having line equation y=0.10246x+0.03990 with correlation coefficient of 0.99925. The developed method was validated as per ICH guide lines with respect to linearity, accuracy (recovery), precision and specificity.The percentage recovery of formoterol fumarate ranged from (98.67±0.2421) and Budesonide ranged from (98.54±0.3330) in tablet dosage form. By treating the data statistically and by recovery study, results of study were validated.