1Dept of Botany and Microbiology, A. V. V. M Sri Pushpam College (Autonomous), Poondi, Thanjavur (Dt) 613 503, Tamil Nadu, India
2Dept of Microbiology, Thanthai Hans Roever College of Arts and Science, Perambalur -621 212, Tamil Nadu, India
*Corresponding Author E-mail: micromadhan@sify.com
Online published on 6 March, 2013.
The chromosomal DNA was isolated from T. harzianum and PCR amplification was performed. The 18S rDNA gene sequencing was made by using ITS (internal transcribed spacer) method. The 18S rDNA gene sequences were submitted to Genebank, NCBI, the accession number GU646678 has been obtained. The phylogenetic tree was constructed using MEGA 4 (Molecular Evolutionary Genetics Analysis Software version 4.0). Based on the neighbour joining tree analysis the isolate T. harzianum has been found closely related to already existing species of Hypocera tixii at 99 per cent of similarity.
Trichoderma harzianum, DNA isolation, Evolution
