1Department of Pharmaceutical Analysis, J. K. K. Nattraja College of Pharmacy, Komarapalayam – 638 183, Namakkal Dt., Tamil Nadu
2Dep. of Pharmaceutical Chemistry, Swamy Vivekanandha College of Pharmacy, Elayampalayam – 637 205, Namakkal Dt., Tamil Nadu
*Corresponding Author E-mail: gbabu73@gmail.com
Online published on 6 March, 2013.
A simple and specific UV derivative spectroscopy and RP-HPLC was developed for buclizine hydrochloride. These methods are carried out by using 234 nm as the wavelength and 100% v/v methanol as solvent. Second derivative spectroscopy was developed to minimize the interferences. The linearity ranges were 10–50 mcg/ml for derivative spectroscopy and 1–10 mcg/ml for the RP-HPLC respectively. Chromatographic separation was made on Phenomenex Luna C18 (250 mm × 4.6 mm, 5μ) column at room temperature with 1.0 ml/min as flow rate and the injection volume was 20 μl. The retention time was found to be 7.15 min. The high recovery and low relative standard deviation confirms the suitability of the methods for the determination of buclizine hydrochloride in pharmaceutical dosage forms.
Buclizine hydrochloride, UV derivative spectroscopy, RP-HPLC, Piperazine derivative, Antiemetic
