Validated HPTLC Method for the Estimation of Antihypertensive Drugs in Pharmaceutical Combined Dosage Forms

A new, rapid, precise, accurate and specific chromatographic methods for the simultaneous determination of Telmisartan and Amlodipine in combined pharmaceutical dosage forms by utilizing silica gel 60F254 high performance thin layer chromatography and densitometric detection at 251nm using chloroform: methanol:formic acid (8.5:1.5:0.5) as the mobile phase. Maximum separation was achieved (Amlodipine Rf 0.47, Telmisartan Rf 0.61) and minimum tailing were obtained and also shows that correlation coefficients were 0.998 for Amlodipine and 0.997 for Telmisartan. The LOD values were 200 ng/spot and 100 ng/spot, while LOQ values were 600 ng/spot and 300 ng/spot for both Amlodipine and Telmisartan respectively. The method was accurate, precise, rapid and found to be suitable for the quantitative analysis of the drug and its dosage form.