1M.R. College of Pharmacy, Phool-Baugh, Vizianagaram, A.P, India
2Roland Institute of Pharmaceutical Sciences, Berhampur, Orissa, India
3Departments of Chemistry, Ravenshaw University, Cuttack, Orissa, India
4Suraksha Pharma Ltd, Suraksh Towers, Hyderabad, A.P, India
*Corresponding Author E-mail: saroj.raul@rediffmail.com
Online published on 7 February, 2013.
A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Olopatadine in pharmaceutical dosage form. Isocratic elution at a flow rate of 1.0 mL min -1 was employed on a Kromasil C18 column at ambient temperature. The mobile phase consisted of methanol: phosphate buffer 60:40 (v/v) and the detection wavelength was at 246 nm. Linearity was observed in concentration range of 10–60 μg/mL. The retention time for Olopatadine was 2.4 min. The method was validated as per the ICH guidelines. The proposed method can be successfully applied for the estimation of Olopatadine in pharmaceutical dosage forms.
Dosage forms, Method development, Validation, Olopatadine, RP-HPLC
