1Dept. of Inorganic Analytical Chemistry, Andhra University, Vishakhapatnam, Andhra Pradesh, India
2Research and Development Dept., Chandra Labs, Hyderabad, Andhra Pradesh, India
3Dept of Pharmaceutical Chemistry, Manipal College of Pharmaceutical Sciences, Karnataka, India
4Dept. of Chemistry, P.R College, Bharathiar University, Tamil Nadu, India
*Corresponding Author E-mail: psranalytical@gmail.com
Online published on 7 February, 2013.
1-(4-Hydrozinophenyl) methyl-1, 2, 4-triazole dihydrochloride (HPT) is an intermediate for synthesis of Rizatriptan. A stability indicating Reverse Phase Ultra High Pressure Liquid Chromatographic (RP-UPLC) method has been developed for the determination of 1-(4-Hydrozinophenyl) methyl-1, 2, 4-triazole Dihydrochloride and its related compounds. The stability-indicating capability of this method has been demonstrated by adequate separation of the degradation products from HPT in stress degraded samples. The separation was achieved on kinetex 1.7 XB-C 18 100 A 50 X 2.1 mm column. The gradient mobile phase consisted mobile phase A 0.01 M potassium dihydrogen phosphate buffer pH 4.5 (adjusted with Orthophosphoric acid) and mobile phase B acetonitrile & methanol in the ratio 80: 20. The method has been demonstrated to be specific, accurate, linear, precise, robust and therefore suitable for routine analysis of HPT.
1-(4-Hydrozinophenyl)methyl-1,2,4-triazole Dihydrochloride, rizatriptan, Reverse Phase Ultra High Pressure Liquid Chromatography, related compounds
