First Order Derivative Spectrophotometric Method for Simultaneous Estimation of Nebivolol Hydrochloride and Indapamide in Tablet Dosage Form

Accurate, precise, rapid and economical spectrophotometric method was developed for the estimation of Indapamide and Nebivolol in bulk dosage form. In first order derivative spectrophotometry, wavelengths selected for quantitation were 271.40nm for Nebivolol (zero crossing point for indapamide) and 244.90nm for Indapamide (zero crossing point for nebivolol). The method was validated as per ICH guideline. In this method the linearity was observed in the concentration range of 6–30μg/ml for Nebivolol and 2–10μg/ml for Indapamide. The accuracy and precision were determined and found to comply with ICH guidelines. The proposed method was successfully applied for the simultaneous estimation of both drugs in commercial tablet preparation.