1Bioanalytical Laboratories, LCRA (A Division of Norwich Clinical Services Pvt. Ltd.), 147/F, 8th Main, 3rd Block, Koramangala, Bangalore -560 034
2Birla Institute of Technology, Mesra, Ranchi -835215, Jharkhand
*Corresponding Author E-mail: arindam.mukhopadhyay@norwichclinical.com
Online published on 7 February, 2013.
Bupropion, hydroxybupropion and fluoxetine, (IS) were extracted from the plasma by LLE method and then separated on a Zorbax SB C8 column at 50°C using the mobile phase mixture of 0.2% formic acid and methanol (35:65, v/v) at a flow rate of 1.0 ml/min. The analytes were detected in API 3000 Mass spectrometer in the positive electrospray ionization mode (split ratio 1.5:1) with multiple reactions monitoring (MRM). The MRM transitions were monitored by following m/z for parent ion 240.1 and daughter ion 184.1 (Bupropion), m/z 256.0 and daughter 238.0 (Hydroxybupropion) and m/z 310.0 and daughter 44.0 (Fluoxetine). A linear calibration plot of bupropion was achieved in the concentration ranges of 1.949 to 270.665 ng/ml and for hydroxybupropion, it was 5.152 to 715.497 ng/ml. Recoveries were consistently more than 60.0%. The assay was specific, precise, accurate and reproducible.
Bupropion, Hydroxybupropion, LCMS/MS, Human plasma, Validation
