Synthesis and Evaluation of Antibacterial Activity of 1, 4-Benzoxazine Analogues

Compound. {(3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl)acetic acid.} and.{(6-chloro-3-oxo-3,4-dihydro-2H-1,4benzoxazin-2-yl)acetic acid.} was synthesized by reacting O-Amino Phenol and Maleic Anhydride. Futher eight Mannich Bases were synthesized by using substituted Aromatic Amines. The structures of Synthesized compounds were confirmed on basis of IR, NMR and Mass Spectra. All synthesized compounds were screened for their Antibacterial Activity against strains viz. E. coli, Staphylococcus aureus, Bacillus sps, Pseudomonas, K.Pneumonea and E. Faecalis. Compounds [3-oxo-4-{[(4-(N-(2-pyridyl)sulfamoyl)phenyl) amino]methyl}-3,4-dihydro-2H-1,4benaoxazin-2-yl] acetic acid, [3-oxo-4-{[(4-(N-(5-methyl-1,2-oxazol-3-yl)sulfamoyl) phenyl) amino]methyl}-3,4dihydro-2H-1,4-benaoxazin-2-yl] acetic acid, [6-chloro-3-oxo-4-{[4-(N-(2-pyridyl)sulfamoylmethyl)phenyl]amino} methyl}-3,4-dihydro-2H-1,4-benzoxazin 2-yl]acetic acid,[6-chloro-3-oxo-4-{[(4-(N-(5-methyl-1,2-oxazol-3-yl) sulfamoylmethyl)phenyl)amino]methyl}-3,4-dihydro-2H-1,4-benzoxazin-2-yl]acetic acid showed good activity against the K.pneumonea and E. faecalis, compounds [3-oxo-4-{[(4-(N-(5-methyl-1,2-oxazol-3-yl)sulfamoyl) phenyl) amino]methyl}-3,4-dihydro-2H-1,4-benaoxazin-2-yl] acetic acid, [6-chloro-3-oxo-4-{[(4-(N-(5-methyl-1,2-oxazol-3yl) sulfamoylmethyl)phenyl)amino]methyl}-3,4-dihydro-2H-1,4-benzoxazin-2-yl]acetic acid showed good activity against the S.aureas, while other compound showed moderate activity against E. coli, klebeseila, Staphy. aureus, E. fecalis. While other derivative shown moderate antibacterial activity.