Quantification of Quercetin and Rutin in Ashwagandharishta by Validated HPTLC Densitometry

Ashwagandharishta is a polyherbal hydro-alcoholic formulation and is used as immunomodulator to promote the health and longevity by increasing defence against disease and also known for its usefulness in the treatment of hypercholesterolemia. A simple, precise and accurate HPTLC method has been established for the determination of quercetin and rutin in Ashwagandharishta–T and Ashwagandharishta-M prepared by traditional and modern methods respectively and also in its marketed formulation. The developed HPTLC method was validated in terms of precision, accuracy, LOD, LOQ and specificity. The amount of quercetin in Ashwagandharishta-T, M and its marketed formulation was found to be 0.0021, 0.00192 and 0.00197% w/w respectively while rutin was found to be 0.00469, 0.00441 and 0.00464% w/w respectively. This is the first report for the quantification of quercetin and rutin in Ashwagandharishta by HPTLC. Furthermore, no TLC densitometric methods have been reported for the quantification of quercetin and rutin from Ashwagandharishta.