RP-HPLC Method Development and Validation for Simultaneous Estimation of Irbesartan and Hydrochlorothiazide in Pharmaceutical Dosage Form

A simple, reproducible and efficient high performance liquid chromatographic method was developed for simultaneous determination of Irbesartan and Hydrochlorothiazide in tablets. A Hypersil pack BDS C18 column 250X4.6 mm 5μ particle size in isocratic mode with mobile phase containing acetonitrile: Buffer (sodium acetate anhydrous) (55:45 v/v) adjusted to pH 3.5 using ortho phosphoric acid. The flow rate was 1.0 ml/min and effluent was monitored at 260 nm. The retention time and linearity range for Irbesartan and Hydrochlorothiazide were (2.98, 4.83 min) and (5–200, 1–400 μg/ml), respectively. The developed method was found to be accurate, precise and selective for simultaneous determination of Irbesartan and Hydrochlorothiazide in tablet dosage form.