Dept. of Biotechnology, Hindustan College of arts and science, Kellambakkam-603 103, Chennai, Tamil Nadu
*Corresponding Author E-mail: lksamy2004@yahoo.com
** E-mail: jayanthi_vijsan@yahoo.co.in
Online published on 7 February, 2013.
The aim of this study was to investigate the effect elevated temperature on A. aceti which converts ethanol to acetic acid in the presence of oxygen. A.aceti was incubated at various temperatures like 37°C, 42°C, and 47°C for overnight. After exposure, the DNA content in each culture was determined. The results showed that an increase in temperature like 42°C and 47°C produced a marked decrease in the DNA content when compared to the culture incubated at 37°C. The expression of gene was confirmed using PCR. The PCR result showed that the gene citrate synthase of A.aceti was expressed in the culture incubated at 37°C and a significant suppression of the gene citrate synthase was seen in the culture treated at 42°C and 47°C.Therefore the elevated temperature on A.aceti will significantly affect the various importance of the organism especially in vinegar production where A.aceti plays a vital role.
A.aceti, elevated temperature, vinegar, citrate synthase gene
