Spectrophotometric method development for Glibanclamide in bulk and Pharmaceutical dosage form using extract of Beta vulgaris root.

A simple, accurate, sensitive and reliable method have been developed for the determination of Glibanclamide in bulk and Pharmaceutical formulation using extract of Beta vulgaris root. Phytochemical investigation shows presence of betanin group of compounds which have polyphenolic nature similar to synthetic dyes like methyl orange, bromocresol green etc. which are commonly used as colouring agent to form a colour complex with amide group containing compounds in developing visible spectroscopic method of analysis. In present work a successful attempt has been done to develop a colorimetric method for estimation of glibanclamide by forming a stable colour complex using extract of Beta vulgaris root on the basis of amide linkage present in glibanclamide. The absorbance of the pale yellow colored complex was measured spectrophotometrically at 533 nm against reagent blank. From Calibration graphs Beer's law is followed in range of 2 to 20μg/ml with correlation coefficient 0.999587, while the LOD and LOQ was 0.27, 0.79 respectively.