Asian Journal of Research In Chemistry
  • Year: 2012
  • Volume: 5
  • Issue: 5

Simultaneous Spectrophotometric Estimation of Tropicamide and Phenylepherin Hydrochloride in Ophthalmic Dosage Form.

  • Author:
  • K.N. Tarkase, S.A. Hapse, P.T. Kadaskar, A.S. Shirsath, N.G. Aher, M.B. Kothule
  • Total Page Count: 3
  • Page Number: 678 to 680

Department of Quality Assurance Technique, Padmashree Dr. Vithalrao Vikhe Patil College of Pharmacy, Ahmednagar. Maharashtra. 414111

*Corresponding Author E-mail: sandiphapse@gmail.com

Online published on 7 February, 2013.

Abstract

A spectrophotometric method for the simultaneous estimation of Tropicamide (TPC) and Phenylepherin hydrochloride (PHE) in their combined dosage form have been developed and validated for linearity, accuracy, precision, ruggedness and repeatability. The wavelength selected to develop the equation were 253 nm and 235 nm in distilled water, For TPC and PHE. Both drugs show linearity in the concentration range of 4–20μg/ml for TPC and 5–125μg/ml for PHE. The results have been statistically validated and were found to be simple, rapid, accurate, precise and reproducible.

Keywords

Tropicamide, phenylepherin hydrochloride, Validation