Simultaneous RP-HPLC Estimation of Moxifloxacin Hydrochloride and Ketorolac Tromethamine in Ophthalmic Dosage Forms

A simple, precise, specific and accurate RP-HPLC method has been developed and Validated for the simultaneous determination of Moxifloxacin Hydrochloride and Ketorolac Tromethamine in Ophthalmic dosage form. The determination was carried out by using Hypersil BDS C18 column (250mm×4.6 mm, 5μm).The Chromatographic separation was done with Acetonitrile: Buffer (pH:4) (60:40) as the mobile phase at a flow rate 1.0 ml/min and the eluent was monitored at 294nm. Buffer solution is prepared by using 0.05 mol Ammonium acetate and pH: 4 is adjusted by using OPA (Ortho Phosphoric Acid). The Retention time of Moxifloxacin Hydrochloride and Ketorolac Tromethamine were 3.54 and 5.62 respectively. Linearity for the Moxifloxacin and Ketorolac were found in the range of 10–90 μg/ml. The method was validated according to the ICH guidelines with respect to specificity, linearity (r²= 0.998), accuracy (102 to 98%), precision and robustness (RSD < 2%). The method was reproducible, with good resolution between Moxifloxacin Hydrochloride and Ketorolac Tromethamine and can be use for routine analysis.