Phytochemical Investigations on Bark and Invitro Microbial Assay of Zanthoxylum armatum DC.

A new amide has been isolated from the Dichloromethane extract of the stem bark of zanthoxylum armatum (Rutaceae) together with four known compounds β-sitosterol, Armatamide, Limonene and Eudesmin. The structure of amide isolated was established as N-(3’,4’-methylene dioxy phenyl ethyl)-3,4-methylene dioxy cinnamoyl amide. Invitro screening experiment for antibacterial activity of dichloromethane bark extract was subjected to biological testing. The minimum inhibitory concentration(MIC-400μg/ML) of extract was determined using standard agar medium by cup and plate method.