Kinetic Spectrophotometric Determination of Antifungal Drugs in Pharmaceuticals

Simple and sensitive kinetic spectrophotometric methods have been developed and validated for the determination of Antifungal drugs, Flucanazole and Ketoconazole in their pharmaceutical dosage forms. The method is based on the oxidation of Flucanazole and Ketoconazole with alkaline and acidic potassium permanganate respectively. In alkaline medium permanganate (Mn⁺⁷) oxidizes Flucanazole and undergoes one electron reduction to give a green colored manganate ions (Mn⁺⁶) which has wavelength maximum at 610 nm, and unreacted permanganate at 526 nm. In acidic medium the course of the reaction was conveniently followed by measuring the absorbance of permanganate at 526 nm, as the permanganate undergoes five electrons reduction to give divalent manganese ions (Mn⁺²). The calibration graphs are linear in the concentration ranges from 30.8–308 μgmL−¹and 20.6 – 206 μgmL−¹using the Fixed time methods and Rate constant methods respectively. The proposed method has a great value in its application to the analysis of Flucanazole and Ketoconazole in quality control laboratories.