Simultaneous Spectrophotometric Estimation of Omeprazole and Ondansetron Hydrochloride by First Order Derivative Spectroscopy Method in Combined Dosage Form.

A simple, accurate and reproducible first order spectrophotometric method, requiring no prior separation, has been developed and validated for the estimation of Omeprazole (OME) and Ondansetron (OND) in combined dosage form. First order derivative spectroscopy method was adopted to eliminate spectral interference. It was developed using wavelengths 309.8 nm and 275 nm as zero crossing points for estimation of OME and OND respectively. The method obeys Beer's Law in concentration range of 5–100μg/ml. The results of analysis have been validated statistically and recovery studies confirmed the accuracy of the proposed method.