Validated HPTLC Method for Analysis of Forskolin in Coleus forskohlii Crude Drug collected from different regions of India

A simple, accurate, selective, precise, economic and rapid high-performance thin-layer chromatographic method for the analysis of forskolin in crude drug form Coleus forskohlii was developed and validated. The method was developed on TLC aluminum plates precoated with silica gel 60F-254 using solvent system benzene: Ethyl acetate (7.5:2.5, v/v), which gives compact spot of forskolin. Densitometric analysis of forskolin was carried out in the absorbance mode at 200 nm. The linear regression analysis data for the calibration plots showed good linear relationship with r = 0.99928 with respect to peak height and peak area respectively in the concentration range 1000– 5000 ng per spot. The limits of detection and quantification were 200 and 600 ng per spot, respectively. The proposed method was applied for determination of forskolin in Coleus forskohlii crude samples. The mean value of correlation coefficient, slope and intercept were 0.9962±0.0015, 87.49±0.68 and 352.41±70, respectively. The method was validated for precision, accuracy and recovery.