Asian Journal of Research In Chemistry
  • Year: 2012
  • Volume: 5
  • Issue: 9

Separation and quantification of Phytohormone production from Methylotrophs (MSF 34) using High performance liquid chromatography

  • Author:
  • Anitha Naren, Vilma P. Sagaai
  • Total Page Count: 5
  • Page Number: 1190 to 1194

Shri AMM Murugappa Chettiar Research Centre, Taramani, Chennai-600 113, India

*Corresponding Author E-mail: anithamcrc@gmail.com

Online published on 7 February, 2013.

Abstract

In this study, intracellular and extracellular phytohormone production was investigated in the methylotroph MSF34. The qualitative and quantitative analysis of these hormones was done using specific bioassays and high performance liquid chromatography (HPLC) technique respectively. Our findings showed that the MSF34 bacteria used in this study synthesized the auxin Indole-3-acetic acid (IAA) and Abscisic acid (ABA). However IAA was found only in microbial spent medium whereas ABA was present in the cell extracts.

Keywords

Extracellular, HPLC, intracellular, methylotrophs, hormone