Selection of Suitable Physico-Chemical Conditions for Gluconic Acid Production by a Mutant Aureobasidium Pullulans SG 80

An experimental study was conducted to improve the production of gluconic acid by the mutant Aureobasidium pullulans SG 80 by optimizing different physic-chemical conditions for fermentation. After optimization of different factors, maximum production was obtained with: glucose, 8%; (NH₄)₂SO₄,1.0%; K₂HPO₄,0.15%; CaCO₃, 0.25%, MnSO₄,,15%; and MgSO₄.7H₂O, 0.25% with maximum utilization of sugar at pH,6.5; volume of medium, 30ml; cell density, 5x106 cells/ml; shaker speed, 200 rpm and temperature, 300C.