Development and Validation of RP-HPLC Method for Simultaneous Estimation of Ramipril and Felodipine in Pharmaceutical Dosage Forms

A simple, rapid, accurate and precise isocratic reversed-phase high-performance liquid chromatographic method has been developed and validated for simultaneous estimation of Ramipril and Felodipine in tablet dosage forms. The chromatographic separation was carried out on Hypersil BDS C18 column (150 x 4.6 mm; 5μ) with a mixture of phosphate buffer pH 5.5: acetonitrile (40: 60 V/V) as a mobile phase; at a flow rate of 1.0 mL/min. UV detection was performed at 243 nm. The retention times were 2.844 min and 5.727 min for Ramipril and Felodipine respectively. Calibration plots were linear (r2=1) over the concentration range of 5–30 μg/mL for both Ramipril and Felodipine. The method was validated for linearity, accuracy, precision, specificity and sensitivity. The proposed method was successfully used for quantitative analysis of Ramipril and Felodipine tablets. No interference from any component of pharmaceutical dosage form was observed. Validation studies revealed that the method is specific, rapid, reliable and reproducible. The high recovery and low relative standard deviation confirm the suitability of the proposed method for routine estimation of Ramipril and Felodipine in bulk and tablet dosage form.