Synthesis of Some Novel 2,5-Disubstituted 1,3,4-Oxadiazole Derivatives as Potential Antibacterial and Anti-inflammatory activity

A novel series of 2-Amidino-5-aryl-1,3,4-oxadiazole derivatives (N1-N4) have been synthesized. A mixture of 2-Amino-5-aryl-1,3,4-oxadiazole, powdered anhydrous aluminium chloride and acetonitrile heated in an oil bath keeping the temperature at 150–160°C for 2 hours to synthesize the derivatives (N₁-N₄). The structures of newly synthesized compounds were established on the basis of FT-IR, ¹H NMR and Mass spectroscopic techniques. The newly synthesized compounds were screened for their in vitro antibacterial activity and in vivo anti-inflammatory activity. In vitro anti-bacterial activity was evaluated by Disc Diffusion technique against Ofloxacin used as standard drug. In vivo anti-inflammatory activity was determined by Carageenan Induced Rat Paw Oedema method against Ibuprofen used as standard drug. Compounds N₁ and N₂ exhibited good antibacterial activity, compound N₄ showed moderate antibacterial activity and compound N₃ showed poor antibacterial activity. Compound N₄ showed good antiinflammatory activity, compounds N₁ and N₃ showed moderate anti-inflammatory activity and compound N₂ showed poor anti-inflammatory activity.