Development and Validation of UV-Spectrophotometric Method for Determination of Cephalexin

To develop simple, sensitive, selective and accurate UV Spectrophotometric methods for the determination of Cephalexin in bulk drug and pharmaceutical formulations (Capsules). The absorbances were measured at 261(Method A) and 257 nm (Method B). The methods was found to be linear from a quantitation ranges of 5μg/ml to 40μg/ml (Method A) in Phosphate Buffer pH 2.0 and 5μg/ml to 50μg/ml (Method B) in 0.1 N HCl. The regression of the curves was Y = 0.020x -0.021 (Method A) and Y = 0.014x -0.102 (Method B). The methods gave satisfactory results in terms of repeatability and intermediate precision (RSD<1.010%) (Method A) and (RSD<0.589%) (Method B) 0.855 μg/mL and 2.85 μg/mL (Method A) and 2.357 μg/mL and 7.857 μg/mL were the LOD and LOQ values, respectively. The methods was validated and proved to be robust and rugged. The results of analysis for these methods have been validated statistically and by recovery studies.