Dipeptidylpeptidase-4 Inhibitory Activity of Pergularia Daemia (Forsk) – An In-vitro Estimation

Dipeptidylpeptidase-4 is an enzyme involved in the inhibition of GLP-1 (Incretin), which is necessary for stimulation of insulin release. It is one of the enzyme targets for the treatment of type-2 diabetes mellitus. Ethanolic and aqueous extracts of Pergularia demia were investigated for activity using ellman method. Successive concentrations (50mg/ml, 100mg/ml, 200 mg/ml, 400 mg/ml, and 800 mg/ml) of ethanolic and aqueous extracts were prepared and investigated for their inhibitory activity towards dipeptidylpeptidase-4 as per Kojima et al., method. It was found that both extracts exhibited significant activity towards DPP-4 inhibition. Diprotein-A was used as standard and distilled water as control.