Reverse Phase High Performance Liquid Chromatographic Estimation of Atazanavir and Ritonavir in Pharmaceutical Dosage Form

A new simple, rapid, specific, accurate, precise and novel reverse phase High Performance Liquid Chromatography (RP-HPLC) method has been developed for the simultaneous estimation of atazanavir and ritonavir in the combined pharmaceutical dosage form. The chromatographic separation for Atazanavir and Ritonavir were achieved with mobile phase containing mixed phosphate buffer (pH 4.0) and acetonitrile (45:55% v/v), Symmetry C18 (4.6 x 100mm, 3.5μm, Make: ACE) at 5°C and UV detection at 237 nm. The compounds were eluted in the isocratic mode at a flow rate of 0.9 ml min⁻¹. The retention times of atazavir 4.29 ± 0.09 min and ritonavir at 5.018± 0.09 min. The above method was validated in terms of linearity, accuracy, precision, LOD, LOQ etc. in accordance with ICH guidelines.