Development and Validation of Multi-Component Mode UV Spectrophotometric Method for the Estimation of Metformin and Glimepiride in Combined Dosage Form

A simple, sensitive, precise and specific UV spectrophotometric method for Multicomponent Mode of Metformin HCl and Metformin in combine dosage form has been developed. The wavelengths 233 nm and 228 nm were selected for the estimation of Metformin HCl and Glimepiride respectively, where Acetonitrile was used as solvent. Linearity were observed in the concentration range of 5–10 μg/ml (r2=0.999) and 3–18 μg/ml (r2=0.999) for Metformin HCl and Glimepiride. These methods were validated according to ICH guidelines.