Chemistry Research Laboratory, Department of Chemistry, B. N. Bandodkar College of Science, Chendani Bunder Road, Thane-400 601. Maharashtra, India
*Corresponding Author E-mail: anitagoswami@yahoo.com
Online published on 10 February, 2015.
DOPA is a precursor of dopamine and acts as a neuro transmitter. Oxidation of it to dopaquinone is a fast reaction by o-diphenol oxidase. Discrepancies in this reaction are responsible for diverse type of disease and disorders. o-diphenol oxidase was purified on natural column and immobilized on agar-Abelmoschus esculentus polymer. Its immobilized o-diphenol oxidase activity showed Vmax =167μmol/lit/min, Km= 2X10−3, Optimum PH = 6.8, Optimum temperature 80°C, Ea = 160.45Kcal/mol and observed highest activity at 45min. behavior of encapsulated activity was compared with free o-diphenol oxidase.
Enzyme-catalyzed reaction, Activation energy of PPO, onion leaves
