Estimation of Voriconazole in Biological Matrix by HPTLC-MS

A High Performance Thin Layer Chromatography Tandem Mass Spectrometry (HPTLC-MS) method was developed for estimation of Voriconazole in a biological matrix. HPTLC-MS is a rapid and economic technique for appropriate identification and quantitation of active pharmaceutical ingredients in pharmaceutical dosage forms and also biological matrices. The Rf value of the drug was 0.33 ± 0.011 using Toluene: Ethyl Acetate: Methanol: (6:0.5:2.5 v/v/v) as the mobile phase. Linearity was obtained within a concentration range of 100 – 600 ng/band with regression coefficient 0.999. The accuracy of the proposed method for analyzing the API in terms of percentage recovery was 100.1 with percentage RSD 1.53%, both of which were within the specified limits, verifying that the method is accurate and precise. LOD and LOQ were 20. 47 ng/band and 62.05 ng/band, respectively. The recovery after application to biological matrix was 97.7± 1.46% proving its applicability to biological analysis. Specificity study showed that the components of biological matrix did not interfere with the analyte, thereby confirming the specificity of the method. Mass spectrometric analysis of the samples resulted in a spectrum showing molecular ion peak at 350.1 m/z. HPTLC-MS method resulted in direct confirmation in identifying the drug. This HPTLC method seems to be convenient as well as less time consuming and thus illustrating its wide applicability.