Chemometrics-assisted Spectrophotometric Determination of Ciprofloxacin and Naphazoline in Eye Drops

Multivariate calibration methods (Classical Least Squares, Inverse Least Squares and Principal Component Analysis) using the absorption spectra in the region of 225 – 265 nm were proposed for the resolution of strongly overlapping spectra of Ciprofloxacin and Naphazoline in binary mixtures. The quantitative predictive abilities of these methods were evaluated by the use of calibration matrix with nine synthetic mixtures of both compounds (24 – 36 mg/L of Ciprofloxacin and 4 – 6 mg/L of Naphazoline). They were also successfully applied to the simultaneous determination of Ciprofloxacin and Naphazoline in commercially available eye drops. Their performances were statistically compared by one-way ANOVA and Bartlett tests clearly indicating their similar accuracy and precision.