Asian Journal of Research In Chemistry

  • Year: 2014
  • Volume: 7
  • Issue: 7

First Order Derivative Spectrophotometric Method for Simultaneous Estimation of Amlodipine Besylate and Indapamide in Combined Pharmaceutical Dosage Form

  • Author:
  • Meghna P. Patel1,, Raj Hasumati2, Nehal Shah3
  • Total Page Count: 4
  • DOI:
  • Page Number: 615 to 618

1Research Scholar, RK University, Rajkot, India

2Assistant Professor, Shree Dhanvantary Pharmacy College, Kim, Gujarat, India

3Principal, Dharmaj Degree Pharmacy College, Dharmaj, Gujarat, India

Abstract

A simple, accurate, precise, rapid, economical UV spectrophotometry method namely first order derivative spectrophotometry have been developed and validated for estimation of Indapamide (IND) and Amlodipine besylate (AML) in combined tablet dosage form and can be used in routine analysis. In this method, the absorbance at 237.4 nm (ZCP of AML) and 241 nm (ZCP of IND) were used for estimation of IND and AML respectively. The method was found to be linear in the concentration range of 1.5–9 μg/ml for IND (r2=0.99983) and 5–30 μg/ml for AML (r2=0.99966). Mean assay was found to be 99.72% and 100.28% for IND and AML respectively.

Keywords

Indapamide (IND), Amlodipine besylate (AML), First order derivative spectrophotometry, Method Development, Validation, ICH guideline