1Department of Chemistry, Faculty of Science, University Putra Malaysia, Malaysia
2Natural Product Laboratory, Faculty of Applied Sciences, Universiti Teknologi MARA, Malaysia
3Hussaini Adamu Federal Polytechnic, Kazaure, Jigawa State, Nigeria
*Corresponding Author E-mail: faujanahmad@gmail.com
Online published on 28 August, 2014.
The enzymatic synthesis of 3-O-β-D-glucopyranoside betulinic acid using Novozyme-435 as a catalyst was studied. The effects of various parameters such as reaction temperature, reaction time and amount of enzymes were investigated. The optimum reaction conditions for the synthesis of 3-O-β-D-glucopyranoside betulinic acid was obtained using 30.67 h, 54.30°C and 180 mg of enzymes using betulinic acid (0.05 mmol) and glucose (0. 1 mmol) respectively. The actual percentage yield under these conditions was 88.69% and the predicted value was 89.05%.
Enzymatic synthesis, 3-O-β-D-glucopyranoside betulinic acid, Novozyme-435 and optimization
