1Chhattisgarh Food and Drug Administration, Mahasamund (CG).
2University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur (C.G.).
3Institute of Pharmacy, Vikram University, Ujjain (MP).
*Corresponding Author E-mail: vishaljain123@gmail.com
Online Published on 21 March, 2023.
Eladi gutika is official in Ayurvedic formulary of India and used as a remedy for Kasa (Cough), Svasa (Asthma), Bhrama (Vertigo), Raktapitta (Bleeding disorders), Jvara (Fever), Amvata (Rheumatism) etc. The present paper is an effort to develop the routine fingerprinting method for quality control parameter of Eladi gutika by high performance thin layer chromatography using glycyrrhizinic acid as an internal standard. The HPTLC estimation was carried out with three laboratory batches and one marketed formulation Eladi gutika and crude drug containing glycyrrhizinic acid. The concentration of glycyrrhizinic acid present in raw material was found to be 8.2201±0.497w/w in G. glabra and in three identical laboratory batch of Eladi gutika EG-I, EG -II and EG -III, was found to be 1.4945 ± 0.293, 1.4963 ± 0.763%, 1.4944± 0.864w/w respectively. The glycyrrhizinic acid content in all the three different batches is found to be in close proximities with each other. The results were comparable to marketed formulations. Hence the present method is simple, sensitive, precise and accurate and can be adopted for routine fingerprinting method for quality control of Eladi gutika.
Eladi gutika, Glycyrrhizinic acid, Ayurveduc formulation, HPTLC, Standardization, Fingerprinting
