Optimization of RP-HPLC for the Accurate Determination of Clindamycin in Bulk and Pharmaceutical Dosage Form

The aim of this research work was to develop and validate a simple, precise, and accurate Reverse Phase High-Performance Liquid Chromatographic (RP-HPLC) method for the estimation of Clindamycin phosphate in bulk and pharmaceutical dosage form. Chromatographic separation was carried out using a Phenomenex C18 column (250mm × 4.6mm, 5.0μm) maintained at 40°C, with a mobile phase consisting of Acetonitrile and 0.05% Ortho Phosphoric Acid in water (30:70 v/v) at a flow rate of 1.0mL/min. The injection volume was 20μL, and detection was performed using a UV detector at 210nm. The run time was 8 minutes. System suitability tests were conducted using a standard solution of Clindamycin (50μg/mL working concentration), and parameters such as relative standard deviation (RSD), tailing factor, and theoretical plates met the acceptance criteria as per pharmacopeial standards. The method showed good repeatability and consistency across five replicate injections. The developed method was found to be robust and reliable for the routine quality control of Clindamycin in pharmaceutical preparations. It ensures effective monitoring of the drug content and complies with validation requirements for linearity, precision, and system suitability.