Isolation and Screening of Antagonistic Effect of Pseudomonas spp.

The present per suit has taken the work to study the antagonistic effect of bacterial population found in soil strata of different districts of Chhattisgarh. After 1: 1000 dilution of soil sample on KMB medium plates, population of Pseudomonas was observed. Preliminarily isolated Pseudomonas sppidentified as Pseudomonas fluorescence through different biochemical tests. To test for antagonistic effect, the soil isolated bacteria were tested against plant pathogenic fungi Sclerotium rolfsii. It was observed that the soil isolated Pseudomonas had antagonistic activity against plant pathogenic fungi Sclerotium rolfsii. Fluorescent Pseudomonas spp. that produce the antibiotic 2,4-diacetylphloroglucinol (DAPG) inhibit a variety of soilborne plant pathogens. Although this preliminary screening is in juvenile stage but it will pave the way for isolation of newer anti metabolites in the form of antibiotics from different strains isolated from soil.