Effect of feeding detoxified waste bread in total mixed ration on nutrient utilization and rumen fermentation pattern in adult buffaloes

The present study was undertaken to detoxify the stale waste bread (WB) and evaluated its nutritional worth in the total mixed ration of adult male buffaloes. The aflatoxin B1 content in the WB before and after detoxification was 55 and 8 ppb, respectively. The in vitro gas production studies revealed that the net gas production (NGP), digestibility of nutrients and availability of metabolizable energy (ME) from WB was higher (P<0.05) than that from maize and wheat. The digestion kinetic parameters for DM and CP assessed by in-sacco technique revealed that the effective and true digestibility was higher (P<0.05) in WB followed by that in wheat and maize grains. For in vivo studies 12 adult male buffaloes (BW 443.0±3.60 kg) divided in to three groups were offered total mixed ration (TMR) containing concentrate mixture in which maize grains were replaced with WB at 0, 50 or 100% on N basis. The complete replacement of maize grains by WB did not show any adverse effect on the daily DM intake, digestibility of nutrients, N-retention, efficiency of N utilization and urinary excretion of purine derivatives in adult male buffaloes. For assessing the impact of these TMRs on the biochemical changes in the rumen, one diet was fed at a time for 30 days to three rumen fistulated male buffaloes. Different N-fractions in the rumen and fluid outflow rate from the rumen were also not affected by the level of WB in the ration of rumen fistulated male buffaloes. It was concluded that detoxified WB can be fed safely and that complete replacement of maize grains in the ration of adult buffaloes did not show any adverse effect on nutrient utilization and health of adult buffaloes.