Tolerance on supplementation of graded levels of novel -mannanase (Natupulse^® TS) in broiler chickens

A study was conducted to evaluate the tolerance of broiler chickens to the β-mannanase (Natupulse^® TS, β-1,4-mananase product), when administered at different levels up to a more than 100-fold of the recommended dose during 35 d. Day-old male broiler chicks (Ross 308 strain) were allocated to five dietary treatments with nine replicates each in RBD. The dietary treatments consisted of a control diet supplemented with ß-mannanaseat 0, 400, 800, 1200 and 100000 TMU/kg feed and were fed in three phases (0-14 d, 14-28 d and 28-35 d of age). During the entire study period (0-35d), the performance parameters (body weight gain, feed intake and feed conversion ratio) did not differ statistically (P>0.05) amongst the dietary treatments. The dietary inclusion of β-mannanase at more than 100-fold of the recommended dose was not affecting performance and mortality of birds during entire growth phase (0-35 d of age). Thus, it was concluded that the β-mannanase administered at 100-fold higher (100000 TMU/kg feed) than recommended dose is well tolerated by broilers chickens and thus, oral supplementation of β-mannanase is safe for poultry.