The use of bio-agents in acclimatizing tissue-cultured plantlets holds great promises in improving micropropagation systems in plants. The bio-agents such as VAM, bacteria and others are reported to substantially enhance the establishment rate of the micropropagated plants. They empower the plants with extra molecular weapons to tackle the situation once exposed to the ambient environment. The main beneficial effects reported are the avoidance of transplanting shock, shorter weaning phase, higher plant growth variates and higher content of total soluble sugars. Moreover, colonization of plants with arbuscular mycorrhizal fungi (AMF) can improve growth by increasing uptake of minerals, improving water relations of the host plant and increasing drought resistance.
